超解像度LIF法を用いた血管内皮細胞表面近傍の物質濃度分布計測
In order to investigate an effect of glycocalyx layer covering vascular endothelial cell surface on molecular transport, a super resolution LIF (Laser Induced Fluorescence) technique for concentration distributions measurement with high spatial resolution based on super resolution microscope, stimulated emission depletion (STED) microscope, was developed. Culturing of endothelial cells on side wall of a microchannel with the width of 500 μm and the depth of 88 μm made from PDMS (Polydimethylsiloxane) was achieved by optimizing surface modification.