Electrophoresis

Abstract(#br)To the best of our knowledge, this paper outlines for the first time high adhesion and hybrid irreversible/reversible microfluidic devices fully composed of polydimethylsiloxane (PDMS). These chips were fabricated by the sandwich bonding (SWB), a method that was recently deployed by our group. SWB offers simple, fast, and low cost operation requiring only a laboratory oven. The devices showed burst pressures of up to 4.5 MPa. This value is more than tenfold the pressures withstood by the full-PDMS chips described in literature.

The micro PIV system has been developed as a new diagnostic tool for microscale flow and applied to the measurement of microchannel flow in order to investigate the properties of electroosmotic flow. The field of view of the PIV system is 580 μm×580μm and velocity vector fields can be measured at 9.3μm vector-to-vector intervals using two-frame cross-correlation method with 32×32 pixels interrogation spot. In this study, two different types of microchips, which are developed for genetic analyses, are employed : PDMS (polymethylmethacrylate) microchip and PMMA (polydimethylsiloxane) microchip.