A Microfluidic System to Evaluate Intestinal Absorption;Original Papers

由 ty10086 提交于 周四, 08/26/2021 - 12:57
文章英文标题
A Microfluidic System to Evaluate Intestinal Absorption;Original Papers
正文
Intestinal absorption rates vary with the nature of the substances involved. In-vitro experiments with cell culture inserts are often conducted to evaluate the intestinal absorption rate. These inserts, however, require large amounts of cells, samples, and culture media, and take a long time to evaluate. To overcome these problems, we developed a microchip-based system that mimics the intestine. The microchip was composed of a glass slide, a permeable membrane, and polydimethylsiloxane (PDMS) sheets, which contained microchannels made by photolithography; Caco-2 cells were cultured on the membrane in the microchip. The system was regulated with a microsyringe pump. We conducted permeation tests; cyclophosphamide, which can permeate the intestinal barrier, displayed a high permeability coefficient and Lucifer yellow, which cannot be absorbed at the intestinal wall, displayed a low permeability coefficient. These results were consistent with those obtained using a conventional method, which supports the validity of our new system. The system realized an 80% reduction of cell consumption.
文章内容（英文）
Intestinal absorption rates vary with the nature of the substances involved. In-vitro experiments with cell culture inserts are often conducted to evaluate the intestinal absorption rate. These inserts, however, require large amounts of cells, samples, and culture media, and take a long time to evaluate. To overcome these problems, we developed a microchip-based system that mimics the intestine. The microchip was composed of a glass slide, a permeable membrane, and polydimethylsiloxane (PDMS) sheets, which contained microchannels made by photolithography; Caco-2 cells were cultured on the membrane in the microchip. The system was regulated with a microsyringe pump. We conducted permeation tests; cyclophosphamide, which can permeate the intestinal barrier, displayed a high permeability coefficient and Lucifer yellow, which cannot be absorbed at the intestinal wall, displayed a low permeability coefficient. These results were consistent with those obtained using a conventional method, which supports the validity of our new system. The system realized an 80% reduction of cell consumption.
来源出处
Journal|[J]Analytical SciencesVolume 25, Issue 12. 2009. PP 1403-1407
DOI
https://doi.org/10.2116/analsci.25.1403

PDMS臻品推荐

PDMS-微流控基质材料-PDMS/道康宁SYLGARD184-聚二甲基硅氧烷/0.5KG(组)

产品说明书PDF自助在线看：http://www.pdmshub.com/sih

信息更新：

美国总工厂生产的0.5KG原始包装的PDMS延期至2021年9月16日后才能交货，默认我们将发国内库存的进口分装产品（02085925-0.5KG）；另受制于有机硅大幅涨价潮影响，2021年9月1日后将上调销售价格，另行通知。Dow Corning=道康宁=Dow SiL=陶氏，都是同一家公司。 2021.09.01更新

美国总工厂生产的0.5KG包装的PDMS恢复正常进口，合法正规，质量金标准，欢迎订购使用。并承诺从我司购买的皆为原厂包装,100%未开封,附带技术手册,非国产灌装仿制、非分装、非仿冒外包装产品,可以放心使用。大量订购更有优惠。2021.04.01更新

备注：由于dowsil与道康宁公司合并，美国总工厂生产的PDMS，价格涨幅高达1倍之多，因针对科研用户使用，特备货规格0.5KG。如果您需要美国总工厂原厂生产的大规格包装的预定，可以与QQ 11366508联系给予报价/货期。2021.05更新

此链接为PDMS-微流控芯片/PDMS/道康宁SYLGARD184/小桶0.5KG装的链接。

一般性的产品性能参数表参考以下小桶装的参数：

微流控芯片/PDMS/道康宁SYLGARD184；微流控芯片/PDMS/道康宁SYLGARD184；微流控芯片/PDMS/道康宁SYLGARD184；

品牌型号：道康宁SYLGARD184

包装规格：0.5KG/罐[含有45.4g固化剂，总重量为0.5KG]

产品颜色：保质期限：36个月

存放环境说明：室温,阴凉处保存

备注说明：

美国道康宁道康宁SYLGARD 184硅橡胶是由液体组分组成的双组分套件产品，包括基本组分与固化剂。基本组分与固化剂按10:1重量比完全混合，中等粘度混合液的稠度与SAE 40机油相似。无论厚薄，混合液将固化成为具有韧性的透明弹性体，最适用于电子／电气方面的封装与灌封应用。 道康宁SYLGARD 184硅橡胶在25~150℃的温度范围内固化，无放热现象，无需二次固化。固化过程完成后，可立即在-55~200℃的温度范围内使用。 产品特性：低毒性，在常规的工业操作中，无特别的注意事项； 无溶剂或固化副产物， 固化时不放热；无需特殊的通风条件，不会产生腐蚀；固化时，收缩量小； 固化后， 透明具有弹性；抗震与减缓机械震动；振动的传递性能小；元件可裸视检查与易修补性； 环保性能；低吸水性，良好的耐辐射性能；高真空状态下的低漏气性； 优异的电性能；较大温度范围内的稳定性， 抗解聚；在-55~200℃范围内，甚至在密闭状态下保持弹性与柔韧性，性能稳定； 阻燃性，UL可燃性分级为94 V-1，温度等级：130℃ 产品用途： 道康宁SYLGARD 184硅橡胶在电气／电子的封装与灌封方面有广泛的应用。