均相磁性PDMS膜的制备以调节细胞行为

In the last decade, polymeric magnetic membranes have been used to investigate mechanical stimuli on adherent cells and cellular mechanotransduction. However, the homogeneity of these particles inside these membranes is often overlooked. In this study, magnetic polydimethylsiloxane (PDMS) membranes were prepared by using magnetite particles (Fe3O4) effectively dispersed inside PDMS for the assessment of possible cellular behavior changes. Magnetite particles were synthesized using co-precipitation method. Magnetic particle embedded PDMS membranes (mpPDMS) were prepared by homogenizing magnetic particles with PDMS elastomer before the curing process. Magnetization, molecular composition, and mechanical properties of these magnetic particles embedded PDMS membranes were analyzed by using Vibrating Sample Magnetometer (VSM), optical microscopy, Fourier Transform Infrared Spectroscopy (FTIR) and rheometry. Biocompatibility, cell viability, and morphology of human fetal osteoblast (hFOB) cells were evaluated by MTT assay, alamarBlue cell proliferation assay and f-actin/DAPI (4,6-diamino-2-phenylindole) staining. Results indicated that membranes fabricated using the presented methodology increased magnetic particle homogeneity compared to previous studies and human fetal osteoblast cells maintained their functionality on 0.5% mpPDMS (w/w) membranes. The methodology for the fabrication of mpPDMS reported here could be used in future studies for the investigation of cellular mechanotransduction pathways in more controlled and repeatable manner on PDMS based substrates.